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ti2e inverted fluorescence microscope system  (Nikon)


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    Nikon ti2e inverted fluorescence microscope system
    ( A to C ) Fraction of nongrowing bacteria upon regrowth from exponential phase (blue), regulated growth arrest (green), or disrupted growth arrest (red), measured using ScanLag ; error bars are SEM over all bacteria within 0.5-hour time bins on at least three biological replicates. (A) Disruption by SHX (0.5 mg/ml). [(B) and (C)] Disrupted growth arrest induced by either (B) CAM (25 μg/ml) or (C) NaN 3 (10.5 mM). The same plots for regulated and exponential cells are reproduced in (B) and (C). ( D to F ) Survival fraction of cultures that were in regulated or disrupted growth arrest and then diluted into fresh medium with ampicillin (100 μg/ml, 6 hours) where disruption was done by (D) SHX, (E) CAM, or (F) NaN 3 . ( G ) Time-lapse microscopy of PI-stained growth-arrested single cells. Cells were either grown to regulated growth arrest or disrupted by SHX for 24 hours and transferred to growth conditions (without PI) under the <t>microscope.</t> Regulated bacteria show significantly lower PI <t>fluorescence,</t> whereas disrupted bacteria may show high PI signal, which disappears upon regrowth. ( H ) Same as in (G), for growth arrest by either CAM or NaN 3 for 24 hours. ( I ) Survival of regulated and disrupted cultures after a treatment with SDS (1%) for 24 hours. This sub-MIC SDS concentration kills only disrupted bacteria (fig. S13). ( J and K ) Treatment with (J) gentamicin (5.2 μg/ml) or (K) vancomycin (1500 μg/ml) for 24 hours during growth arrest is only effective against disrupted conditions with active protein production (Mann-Whitney, * P ≤ 0.05; ** P ≤ 0.005).
    Ti2e Inverted Fluorescence Microscope System, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 57094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti2e+inverted+fluorescent+microscope/pmc12758538-188-6-5?v=Nikon
    Average 99 stars, based on 57094 article reviews
    ti2e inverted fluorescence microscope system - by Bioz Stars, 2026-07
    99/100 stars

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    1) Product Images from "Differentiation between regulated and disrupted growth arrests allows tailoring of effective treatments for antibiotic persistence"

    Article Title: Differentiation between regulated and disrupted growth arrests allows tailoring of effective treatments for antibiotic persistence

    Journal: Science Advances

    doi: 10.1126/sciadv.adt6577

    ( A to C ) Fraction of nongrowing bacteria upon regrowth from exponential phase (blue), regulated growth arrest (green), or disrupted growth arrest (red), measured using ScanLag ; error bars are SEM over all bacteria within 0.5-hour time bins on at least three biological replicates. (A) Disruption by SHX (0.5 mg/ml). [(B) and (C)] Disrupted growth arrest induced by either (B) CAM (25 μg/ml) or (C) NaN 3 (10.5 mM). The same plots for regulated and exponential cells are reproduced in (B) and (C). ( D to F ) Survival fraction of cultures that were in regulated or disrupted growth arrest and then diluted into fresh medium with ampicillin (100 μg/ml, 6 hours) where disruption was done by (D) SHX, (E) CAM, or (F) NaN 3 . ( G ) Time-lapse microscopy of PI-stained growth-arrested single cells. Cells were either grown to regulated growth arrest or disrupted by SHX for 24 hours and transferred to growth conditions (without PI) under the microscope. Regulated bacteria show significantly lower PI fluorescence, whereas disrupted bacteria may show high PI signal, which disappears upon regrowth. ( H ) Same as in (G), for growth arrest by either CAM or NaN 3 for 24 hours. ( I ) Survival of regulated and disrupted cultures after a treatment with SDS (1%) for 24 hours. This sub-MIC SDS concentration kills only disrupted bacteria (fig. S13). ( J and K ) Treatment with (J) gentamicin (5.2 μg/ml) or (K) vancomycin (1500 μg/ml) for 24 hours during growth arrest is only effective against disrupted conditions with active protein production (Mann-Whitney, * P ≤ 0.05; ** P ≤ 0.005).
    Figure Legend Snippet: ( A to C ) Fraction of nongrowing bacteria upon regrowth from exponential phase (blue), regulated growth arrest (green), or disrupted growth arrest (red), measured using ScanLag ; error bars are SEM over all bacteria within 0.5-hour time bins on at least three biological replicates. (A) Disruption by SHX (0.5 mg/ml). [(B) and (C)] Disrupted growth arrest induced by either (B) CAM (25 μg/ml) or (C) NaN 3 (10.5 mM). The same plots for regulated and exponential cells are reproduced in (B) and (C). ( D to F ) Survival fraction of cultures that were in regulated or disrupted growth arrest and then diluted into fresh medium with ampicillin (100 μg/ml, 6 hours) where disruption was done by (D) SHX, (E) CAM, or (F) NaN 3 . ( G ) Time-lapse microscopy of PI-stained growth-arrested single cells. Cells were either grown to regulated growth arrest or disrupted by SHX for 24 hours and transferred to growth conditions (without PI) under the microscope. Regulated bacteria show significantly lower PI fluorescence, whereas disrupted bacteria may show high PI signal, which disappears upon regrowth. ( H ) Same as in (G), for growth arrest by either CAM or NaN 3 for 24 hours. ( I ) Survival of regulated and disrupted cultures after a treatment with SDS (1%) for 24 hours. This sub-MIC SDS concentration kills only disrupted bacteria (fig. S13). ( J and K ) Treatment with (J) gentamicin (5.2 μg/ml) or (K) vancomycin (1500 μg/ml) for 24 hours during growth arrest is only effective against disrupted conditions with active protein production (Mann-Whitney, * P ≤ 0.05; ** P ≤ 0.005).

    Techniques Used: Bacteria, Disruption, Time-lapse Microscopy, Staining, Microscopy, Fluorescence, Concentration Assay, MANN-WHITNEY



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    ( A to C ) Fraction of nongrowing bacteria upon regrowth from exponential phase (blue), regulated growth arrest (green), or disrupted growth arrest (red), measured using ScanLag ; error bars are SEM over all bacteria within 0.5-hour time bins on at least three biological replicates. (A) Disruption by SHX (0.5 mg/ml). [(B) and (C)] Disrupted growth arrest induced by either (B) CAM (25 μg/ml) or (C) NaN 3 (10.5 mM). The same plots for regulated and exponential cells are reproduced in (B) and (C). ( D to F ) Survival fraction of cultures that were in regulated or disrupted growth arrest and then diluted into fresh medium with ampicillin (100 μg/ml, 6 hours) where disruption was done by (D) SHX, (E) CAM, or (F) NaN 3 . ( G ) Time-lapse microscopy of PI-stained growth-arrested single cells. Cells were either grown to regulated growth arrest or disrupted by SHX for 24 hours and transferred to growth conditions (without PI) under the <t>microscope.</t> Regulated bacteria show significantly lower PI <t>fluorescence,</t> whereas disrupted bacteria may show high PI signal, which disappears upon regrowth. ( H ) Same as in (G), for growth arrest by either CAM or NaN 3 for 24 hours. ( I ) Survival of regulated and disrupted cultures after a treatment with SDS (1%) for 24 hours. This sub-MIC SDS concentration kills only disrupted bacteria (fig. S13). ( J and K ) Treatment with (J) gentamicin (5.2 μg/ml) or (K) vancomycin (1500 μg/ml) for 24 hours during growth arrest is only effective against disrupted conditions with active protein production (Mann-Whitney, * P ≤ 0.05; ** P ≤ 0.005).
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    Image Search Results


    ( A to C ) Fraction of nongrowing bacteria upon regrowth from exponential phase (blue), regulated growth arrest (green), or disrupted growth arrest (red), measured using ScanLag ; error bars are SEM over all bacteria within 0.5-hour time bins on at least three biological replicates. (A) Disruption by SHX (0.5 mg/ml). [(B) and (C)] Disrupted growth arrest induced by either (B) CAM (25 μg/ml) or (C) NaN 3 (10.5 mM). The same plots for regulated and exponential cells are reproduced in (B) and (C). ( D to F ) Survival fraction of cultures that were in regulated or disrupted growth arrest and then diluted into fresh medium with ampicillin (100 μg/ml, 6 hours) where disruption was done by (D) SHX, (E) CAM, or (F) NaN 3 . ( G ) Time-lapse microscopy of PI-stained growth-arrested single cells. Cells were either grown to regulated growth arrest or disrupted by SHX for 24 hours and transferred to growth conditions (without PI) under the microscope. Regulated bacteria show significantly lower PI fluorescence, whereas disrupted bacteria may show high PI signal, which disappears upon regrowth. ( H ) Same as in (G), for growth arrest by either CAM or NaN 3 for 24 hours. ( I ) Survival of regulated and disrupted cultures after a treatment with SDS (1%) for 24 hours. This sub-MIC SDS concentration kills only disrupted bacteria (fig. S13). ( J and K ) Treatment with (J) gentamicin (5.2 μg/ml) or (K) vancomycin (1500 μg/ml) for 24 hours during growth arrest is only effective against disrupted conditions with active protein production (Mann-Whitney, * P ≤ 0.05; ** P ≤ 0.005).

    Journal: Science Advances

    Article Title: Differentiation between regulated and disrupted growth arrests allows tailoring of effective treatments for antibiotic persistence

    doi: 10.1126/sciadv.adt6577

    Figure Lengend Snippet: ( A to C ) Fraction of nongrowing bacteria upon regrowth from exponential phase (blue), regulated growth arrest (green), or disrupted growth arrest (red), measured using ScanLag ; error bars are SEM over all bacteria within 0.5-hour time bins on at least three biological replicates. (A) Disruption by SHX (0.5 mg/ml). [(B) and (C)] Disrupted growth arrest induced by either (B) CAM (25 μg/ml) or (C) NaN 3 (10.5 mM). The same plots for regulated and exponential cells are reproduced in (B) and (C). ( D to F ) Survival fraction of cultures that were in regulated or disrupted growth arrest and then diluted into fresh medium with ampicillin (100 μg/ml, 6 hours) where disruption was done by (D) SHX, (E) CAM, or (F) NaN 3 . ( G ) Time-lapse microscopy of PI-stained growth-arrested single cells. Cells were either grown to regulated growth arrest or disrupted by SHX for 24 hours and transferred to growth conditions (without PI) under the microscope. Regulated bacteria show significantly lower PI fluorescence, whereas disrupted bacteria may show high PI signal, which disappears upon regrowth. ( H ) Same as in (G), for growth arrest by either CAM or NaN 3 for 24 hours. ( I ) Survival of regulated and disrupted cultures after a treatment with SDS (1%) for 24 hours. This sub-MIC SDS concentration kills only disrupted bacteria (fig. S13). ( J and K ) Treatment with (J) gentamicin (5.2 μg/ml) or (K) vancomycin (1500 μg/ml) for 24 hours during growth arrest is only effective against disrupted conditions with active protein production (Mann-Whitney, * P ≤ 0.05; ** P ≤ 0.005).

    Article Snippet: Microscopy was done using a Nikon Ti2E inverted fluorescence microscope system, ×100 NA (numerical aperture) 1.45 oil objective with phase-contrast, automated stage and shutters, and automated focus hardware [Perfect Focus System (PFS)].

    Techniques: Bacteria, Disruption, Time-lapse Microscopy, Staining, Microscopy, Fluorescence, Concentration Assay, MANN-WHITNEY